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to address if serpina2 was accumulating in the er, we carried out double-labeling assays against the v5-tag and two er markers, calnexin canx or protein disulfide-isomerase a3 pdia3 .

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biomarkers such as serpina-1 polypeptides and albumin polypeptides are preferably captured with capture reagents immobilized to a solid support, such as any biochip described herein, a multiwell microtiter plate, a resin, or other suitable support.

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the second mechanism is that serpina3n expression inside the cell, within endosomes and vesicles, blocks the activity of select proteases that could be cleaving the membrane stabilizing complexes from inside the cell.


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